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My male was castrated last Wednesday 5 days ago, and my girl has now come on heat. Do I need to keep them separated or his he fully sterile by now?

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What did the vet tell you .I gather you asked?
But i would imagine its like the human version where there is a period to wait .
Besides the other obvious if he was only spayed last week he should not be allowed to even try mating her with the stitches in & that common sense after surgery protocol is followed 

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